Meridian’s 55C MMLV-RT is a highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.
Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However, for cDNA synthesis, a higher reaction temperature is sometimes desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimises nonspecific primer binding. 55C MMLV-RT is a highly thermostable reverse transcriptase with reduced RNase H activity, that allows very efficient first-strand cDNA synthesis at temperatures up to 60°C. This improves the cDNA yield from difficult RNA targets, such as viral RNA genomes, that require higher temperature to denature strong RNA secondary structure.
- Thermostable reverse transcriptase activity up to 60°C
- Ideal for cDNA synthesis from RNA with high secondary structure such as viral RNA genomes
- Sensitive detection of low copy number RNA targets
- Perfect for developing fast, highly reproducible RT-qPCR assays
This product has met the following criteria to qualify for the following awards:
 | Sustainable Distribution & Packaging | - Country of origin has an Environmental Performance Index (EPI) greater than 60
|
To find out more about our Sustainability Attributes, click here.